1. Concentration

-         Molar, %, (wt/vol, vol/vol)

2. Enzyme reaction setup

-         Restriction

-         Ligation

-         Etc.

3. Plasmids: general features or characteristics

4. Transformation: factors critical to bacterial transformation, etc.

5. Quantitation of DNA or RNA using O.D.

6. Calculation of OD to concentration (ug/ml)

7. PCR

-         phases

-         general profiles (cycle)

-         reaction components

-         reaction setup

8. Genotype frequency calculation

9. Allele frequency calculation